Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315
Alternative Synonym:
Follistatin, FS, FST, Active-binding protein
Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (Use in combination with clone FST/4282 for two-site ELISA), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
SDS-PAGE Analysis of Purified Follistatin Mouse Monoclonal Antibody (FST/4281). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human testis stained with Follistatin Mouse Monoclonal Antibody (FST/4281).
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