Tuberculin conjugated synthetic peptide (LLSHVRHARYAP), derived from the C-terminus of human Estrogen Receptor beta 5
Alternative Synonym:
Estrogen Receptor 2, Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2), Oestrogen Receptor Beta, Estrogen Receptor Beta, ER-BETA, ESTRB
This MAb specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). No cross-reactivity is seen with estrogen receptor beta 1 or 2 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunofluorescence (2-4ug/ml), Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed
SDS-PAGE Analysis of Purified ER-beta5 Mouse Monoclonal Antibody (PPG5/25). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human breast stained with ER-beta5 Mouse Monoclonal Antibody (PPG5/25).
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