This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA
Western Blot Analysis of human Intestine
Western Blot Analysis of A-549 lysate using Cytokeratin 18 Mouse Monoclonal Antibody (DC10)
Western Blot Analysis (1) HeLa and (2) A431 lysate using Cytokeratin 18 Mouse Monoclonal Antibody (DC10).
Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 18 Mouse Monoclonal Antibody (DC10).
Western Blot Analysis of human Intestine tissue lysate using Cytokeratin 18 Mouse Monoclonal Antibody (DC10).
Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (DC10).
SDS-PAGE Analysis of Purified Cytokeratin 18 Mouse Monoclonal Antibody (DC10). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of A549, HT-29, HCT-116, A-431, K562 and MCF7 cell lysates using Cytokeratin 18 Mouse Monoclonal Antibody (DC10)
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