B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p
Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alphaheavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alphaheavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alphaheavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with B2M Rabbit Recombinant Monoclonal Antibody (B2M/1857R).
SDS-PAGE Analysis PurifiedB2M Rabbit Recombinant Monoclonal Antibody (B2M/1857R). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of human HeLa cells using Beta-2-Microglobulin Rabbit Recombinant Monoclonal Antibody (B2M/1857R) followed by goat anti-mouse IgG-CF488 (Blue), Isotype control (Red).
Western Blot Analysis of THP-1 and Raji cell lysate using B2M Rabbit Recombinant Monoclonal Antibody (B2M/1857R).
Immunofluorescence Analysis of PFA fixed HeLa cells labeling with Beta-2-Microglobulin Rabbit Recombinant Monoclonal Antibody (B2M/1857R), followed by goat anti-rabbit IgG-CF488 (Green).
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain,Human Lungs, Mouse Lungs and Rat Lungs tissue lysates using Beta-2 Microglobulin Rabbit Recombinant Monoclonal Antibody (B2M/1857R).
Western Blot Analysis of Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach and Human Testis tissue lysates using Carbonic Anhydrase IX Recombinant Rabbit Monoclonal Antibody (CA9/9621R).
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