Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1).
Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).
Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Monoclonal Antibody (UCHL-
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1).
Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).
Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Monoclonal Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue), Isotype Control (Red).
SDS-PAGE Analysis of Purified CD45RO MAb (UCHL-1). Confirmation of Integrity and Purity of Antibody
Immunofluorescence Analysis of human tonsil FFPE section stained with anti-CD45RO (UCHL-1) and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
