PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Western Blot (1-2ug/ml),,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, p
Fo
Formalin-fixed, paraffin-embedded human Hodgkin&apos,s Lymphoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).
Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).
Formalin-fixed, paraffin-embedded human Spleen stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).
Western Blot Analysis of THP-1 cell lysate using PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).
SDS-PAGE Analysis Purified PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1 Mouse Monoclonal Antibody (PU1/2146) followed by goat anti-mouse IgG-CF488 (Green). Membrane is labeled with Phalloiden (Red).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
