It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
SDS-PAGE Analysis of Purified CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R).
Immunofluorescence Analysis of K562 cells labeling
Formalin-fixed, paraffin-embedded human Tonsil stained with CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R).
SDS-PAGE Analysis of Purified CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R).
Immunofluorescence Analysis of K562 cells labeling CD43 with CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R) followed by Goat anti-Rabbit- IgG-CF488 (Green). The nuclear counterstain is NucSpotvÜ.
Western Blot Analysis of K562 cell lysate using CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R).
Flow Cytometric Analysis of PFA-fixed K562 cells using CD43 Rabbit Recombinant Monoclonal Antibody (SPN/1766R) followed by Goat anti-Rabbit- IgG-CF488 (Blue), Isotype Control (Red)
Western Blot Analysis of HL-60 cell lysate using CD43 Recombinant Rabbit Monoclonal Antibody (SPN/1766R).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
