Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Formalin-fixed, paraffin-e
Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).
Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).
Formalin-fixed, paraffin-embedded Mouse Kidney stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).
Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Western Blot Analysis of HeLa cell lysate using Spectrin beta III Monoclonal Antibody (SPTBN2/1584).
SDS-PAGE Analysis Purified Spectrin beta III Monoclonal Antibody (SPTBN2/1584). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of PANC-1 cell lysate using Spectrin beta III Monoclonal Antibody (SPTBN2/1584).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
