Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Western Blot (1-2ug/ml),Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed ti
Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta III Recombinant Rabbit Monoclonal (SPTBN2/3142R).
Western Blot Analysis of HeLa, PC-3 cells and Brain tissue lysate using Spectrin beta III Recombinant Rabbit Monoclonal (SPTBN2/3142R).
Immunofluorescence Analysis of HeLa cells labeling Spectrin Beta III with Spectrin beta III Recombinant Rabbit Monoclonal (SPTBN2/3142R) followed by Goat anti-Mouse IgG-CF488 (Green). Nuclear counterstain is Reddot (Red)
SDS-PAGE Analysis Purified Spectrin beta III Recombinant Rabbit Monoclonal (SPTBN2/3142R). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of HeLa cells using Spectrin beta III Recombinant Rabbit Monoclonal (SPTBN2/3142R) followed by Goat anti-rabbit IgG-CF488 (Blue), Isotype Control (Red).
Western Blot Analysis of Kidney and Testis tissue lysates of different species using Spectrin Beta 3 Recombinant Rabbit Monoclonal Antibody (SPTBN2/3142R).
Western Blot Analysis of Human Kidney, Human Testis and HeLa lysates using Spectrin Beta 3 Recombinant Rabbit Monoclonal Antibody (SPTBN2/3142R).
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