GRP78 / BiP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-13-101
- Bilder (7)
| Artikelname: | GRP78 / BiP Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-13-101 |
| Hersteller Artikelnummer: | 13-101 |
| Alternativnummer: | PRS-13-101-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using HSPA5 antibody (13-101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
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Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining. |
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