PARP1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-13-327
- Bilder (6)
| Artikelname: | PARP1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-13-327 |
| Hersteller Artikelnummer: | 13-327 |
| Alternativnummer: | PRS-13-327-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200CHIP: 1:50 - 1:200 |
|
|
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-327) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
|
|
Western blot analysis of extracts of Jurkat cells, using PARP1 antibody (13-327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
|
|
Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody (13-327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
|
|
Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody (13-327) at dilution of 1:200 (40x lens). |
|
|
Immunohistochemistry of paraffin-embedded rat heart using PARP1 antibody (13-327) at dilution of 1:200 (40x lens). |
|
|
Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody (13-327) at dilution of 1:200 (40x lens). |
Produktgarantie und fachkundiger Support
