YAP1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-370
Artikelname: YAP1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-370
Hersteller Artikelnummer: 13-370
Alternativnummer: PRS-13-370-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
Konjugation: Unconjugated
Alternative Synonym: YAP1, YAP, YAP2, YAP65, YKI
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 72kDa
NCBI: 10413
UniProt: P46937
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using YAP1 antibody (13-370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody (13-370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (13-370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using YAP1 antibody (13-370) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using YAP1 antibody (13-370) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody (13-370) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using PARP1 antibody (13-327) at dilution of 1:100. Blue: DAPI for nuclear staining.