CLUH Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-454
Artikelname: CLUH Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-454
Hersteller Artikelnummer: 13-454
Alternativnummer: PRS-13-454-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1120-1309 of human CLUH (NP_056044.3).
Konjugation: Unconjugated
Alternative Synonym: CLU1, clustered mitochondria protein homolog, clustered mitochondria (cluA/CLU1) homolog
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 180kDa
NCBI: 23277
UniProt: O75153
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CLUH antibody (13-454) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human placenta using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.