TPD52 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-535
Artikelname: TPD52 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-535
Hersteller Artikelnummer: 13-535
Alternativnummer: PRS-13-535-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human TPD52 (NP_005070.1).
Konjugation: Unconjugated
Alternative Synonym: TPD52, tumor protein D52, D52, N8L, PC-1, PrLZ, hD52, PC1/MRPS28 fusion protein, prostate and colon associated protein
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 26kDa
NCBI: 7163
UniProt: P55327
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using TPD52 antibody (13-535) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human mammary cancer using TPD52 antibody (13-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TPD52 antibody (13-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TPD52 antibody (13-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using TPD52 antibody (13-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using TPD52 antibody (13-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TPD52 antibody (13-535) at dilution of 1:100 (40x lens).