EGLN1/EGLN2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-591
Artikelname: EGLN1/EGLN2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-591
Hersteller Artikelnummer: 13-591
Alternativnummer: PRS-13-591-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide of human EGLN1/EGLN2
Konjugation: Unconjugated
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 44kDa
NCBI: 54583
UniProt: Q9GZT9
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EGLN1/EGLN2 antibody (13-591) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EGLN1+EGLN2 antibody (13-591) at dilution of 1:100 (40x lens).