SDHB Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-817
Artikelname: SDHB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-817
Hersteller Artikelnummer: 13-817
Alternativnummer: PRS-13-817-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_002991.2).
Konjugation: Unconjugated
Alternative Synonym: SDHB, IP, PGL4, SDH, SDH1, SDHIP, CWS2, SDH2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 32kDa
NCBI: 6390
UniProt: P21912
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using SDHB antibody (13-817) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon using SDHB antibody (13-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SDHB antibody (13-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SDHB antibody (13-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SDHB antibody (13-817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using SDHB antibody (13-817) at dilution of 1:100 (40x lens).