Iba1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-395
Artikelname: Iba1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-395
Hersteller Artikelnummer: 14-395
Alternativnummer: PRS-14-395-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3).
Konjugation: Unconjugated
Alternative Synonym: AIF1, allograft inflammatory factor 1, IBA1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, G1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 16kDa
NCBI: 199
UniProt: P55008
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of rat spleen, using Iba1 antibody (14-395) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).&x0D,
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