PGK2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-721
Artikelname: PGK2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-721
Hersteller Artikelnummer: 14-721
Alternativnummer: PRS-14-721-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).
Konjugation: Unconjugated
Alternative Synonym: PGK2, PGK-2, PGKB, PGKPS, dJ417L20.2, HEL-S-272
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 45kDa
NCBI: 5232
UniProt: P07205
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using PGK2 antibody (14-721) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (14-721) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using PGK2 Polyclonal Antibody (14-721) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.