PRKCB Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-109
Artikelname: PRKCB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-109
Hersteller Artikelnummer: 15-109
Alternativnummer: PRS-15-109-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PRKCB (NP_002729.2).
Konjugation: Unconjugated
Alternative Synonym: PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 77kDa
NCBI: 5579
UniProt: P05771
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of mouse liver, using PRKCB antibody (15-109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 50s.
Immunohistochemistry of paraffin-embedded mouse lung using PRKCB antibody (15-109) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PRKCB antibody (15-109) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using PRKCB Polyclonal Antibody (15-109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using PRKCB Polyclonal Antibody (15-109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using PRKCB Polyclonal Antibody (15-109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.