G3BP1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-756
Artikelname: G3BP1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-756
Hersteller Artikelnummer: 15-756
Alternativnummer: PRS-15-756-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 131-330 of human G3BP1 (NP_938405.1).
Konjugation: Unconjugated
Alternative Synonym: G3BP, G3BP, HDH-VIII
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 68kDa
NCBI: 10146
UniProt: Q13283
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using G3BP1 antibody (15-756) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).