HSPB1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-16-557
Artikelname: HSPB1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-16-557
Hersteller Artikelnummer: 16-557
Alternativnummer: PRS-16-557-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
Konjugation: Unconjugated
Alternative Synonym: HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 25kDa
NCBI: 3315
UniProt: P04792
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (16-557) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB1 antibody (16-557) at dilution of 1:100. Blue: DAPI for nuclear staining.