MLKL Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-16-911
Artikelname: MLKL Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-16-911
Hersteller Artikelnummer: 16-911
Alternativnummer: PRS-16-911-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein of mouse MLKL
Konjugation: Unconjugated
Alternative Synonym: MLKL
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 54kDa
NCBI: 197259
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MLKL antibody (16-911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using MLKL antibody (16-911) at dilution of 1:200 (40x lens).