FKBP5 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-189
Artikelname: FKBP5 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-189
Hersteller Artikelnummer: 19-189
Alternativnummer: PRS-19-189-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 308-457 of human FKBP5 (NP_001139247.1).
Konjugation: Unconjugated
Alternative Synonym: AIG61, FKBP54, P54, PPIase, Ptg-1FKBP5, peptidyl-prolyl cis-trans isomerase FKBP5, 51 kDa FK506-binding protein, 51 kDa FKBP, 54 kDa progesterone receptor-associated immunophilin, FF1 antigen, FK506 binding protein 5, FKBP-51, HSP90-binding immunophilin, PPIase FKBP5, T-cell FK506-binding protein, androgen-regulated protein 6, peptidylprolyl cis-trans isomerase, rotamase
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 51kDa
NCBI: 2289
UniProt: Q13451
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using FKBP5 antibody (19-189) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon using FKBP5 antibody (19-189) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using FKBP5 antibody (19-189) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using FKBP5 antibody (19-189) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FKBP5 antibody (19-189) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FKBP5 antibody (19-189) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using FKBP5 antibody (19-189) at dilution of 1:100. Blue: DAPI for nuclear staining.