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IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal

IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-372

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Artikelname:	IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-19-372
Hersteller Artikelnummer:	19-372
Alternativnummer:	PRS-19-372-100
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, IP, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
Konjugation:	Unconjugated
Alternative Synonym:	CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IGF2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, cancer/testis antigen 98

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 70kDa
NCBI:	10643
UniProt:	O00425
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

	Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (19-372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (19-372) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (19-372). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (19-372) at a dilition of 1:1000.

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