STXBP1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-651
Artikelname: STXBP1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-651
Hersteller Artikelnummer: 19-651
Alternativnummer: PRS-19-651-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat, Zebrafish
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1).
Konjugation: Unconjugated
Alternative Synonym: STXBP1, MUNC18-1, UNC18, hUNC18, rbSec1, P67, NSEC1, RBSEC1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 68kDa
NCBI: 6812
UniProt: P61764
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using STXBP1 antibody (19-651) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.