Western blot analysis of extracts of various cell lines, using RFC4 antibody (19-694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody (19-694). Western blot was performed from the immunoprecipitate using RFC4 antibody (19-694) at a dilition of 1:1000.
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