FKBP4 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-774
Artikelname: FKBP4 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-774
Hersteller Artikelnummer: 19-774
Alternativnummer: PRS-19-774-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-459 of human FKBP4 (NP_002005.1).
Konjugation: Unconjugated
Alternative Synonym: FKBP4, FK506 binding protein 4, 59 kDa, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52, FK506 binding protein 4 (59kD), FK506-binding protein 4, FK506-binding protein 4 (59kD), HSP binding immunophilin, T-cell FK506-binding protein, 59kD, p59 protein, peptidylpro
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 58kDa
NCBI: 2288
UniProt: Q02790
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using FKBP4 antibody (19-774) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human gastric cancer using FKBP4 Antibody (19-774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using FKBP4 Antibody (19-774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using FKBP4 Antibody (19-774) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using FKBP4 antibody (19-774). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug FKBP4 antibody (19-774). Western blot was performed from the immunoprecipitate using FKBP4 antibody (19-774) at a dilition of 1:1000.