53BP1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-19-839
- Bilder (6)
| Artikelname: | 53BP1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-19-839 |
| Hersteller Artikelnummer: | 19-839 |
| Alternativnummer: | PRS-19-839-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1 (NP_001135452.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | TP53BP1, 53BP1, p202, p53-binding protein 1, p53-binding protein 53bp1, p53BP1 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of A-431 cells, using 53BP1 antibody (19-839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 40s. |
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Immunohistochemistry of paraffin-embedded rat kidney using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human lung cancer using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse heart using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of U2OS cells using 53BP1 antibody (19-839). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using 53BP1 antibody (19-839). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region. |
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