RPS3A Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-919
Artikelname: RPS3A Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-919
Hersteller Artikelnummer: 19-919
Alternativnummer: PRS-19-919-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
Konjugation: Unconjugated
Alternative Synonym: 40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255|HAMAP-Rule:MF_03122}, FTE1, MFTL
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 35kDa
NCBI: 6189
UniProt: P61247
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (19-919). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug RPS3A antibody (19-919). Western blot was performed from the immunoprecipitate using RPS3A antibody (19-919) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using RPS3A antibody (19-919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).