GAB1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-081
Artikelname: GAB1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-081
Hersteller Artikelnummer: 22-081
Alternativnummer: PRS-22-081-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 485-724 of human GAB1 (NP_997006.1).
Konjugation: Unconjugated
Alternative Synonym: ADDA, Growth factor receptor bound protein 2-associated protein 1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 76kDa
NCBI: 2549
UniProt: Q13480
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100
Western blot analysis of extracts of various cell lines, using GAB1 antibody (22-081) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 80s.
Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GAB1 antibody (22-081). Blue: DAPI for nuclear staining.