Western blot analysis of extracts of various cell lines, using GAB1 antibody (22-081) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 80s.
Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GAB1 antibody (22-081). Blue: DAPI for nuclear staining.
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