CIRBP Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-312
Artikelname: CIRBP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-312
Hersteller Artikelnummer: 22-312
Alternativnummer: PRS-22-312-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1).
Konjugation: Unconjugated
Alternative Synonym: CIRBP, CIRP
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 18kDa
NCBI: 1153
UniProt: Q14011
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using CIRBP antibody (22-312) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody (22-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody (22-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CIRBP antibody (22-312) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CIRBP antibody (22-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CIRBP antibody (22-312) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CIRBP antibody (22-312). Blue: DAPI for nuclear staining.