GLUD2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-344
Artikelname: GLUD2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-344
Hersteller Artikelnummer: 22-344
Alternativnummer: PRS-22-344-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 319-558 of human GLUD2 (NP_036216.2).
Konjugation: Unconjugated
Alternative Synonym: GLUD2, GDH2, GLUDP1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 55kDa
NCBI: 2747
UniProt: P49448
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using GLUD2 antibody (22-344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using GLUD2 antibody (22-344) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using GLUD2 antibody (22-344) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using GLUD2 antibody (22-344) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GLUD2 antibody (22-344) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using GLUD2 antibody (22-344). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using GLUD2 antibody (22-344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.