EIF3J Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-694
Artikelname: EIF3J Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-694
Hersteller Artikelnummer: 23-694
Alternativnummer: PRS-23-694-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).
Konjugation: Unconjugated
Alternative Synonym: EIF3S1, eIF3-alpha, eIF3-p35, eukaryotic translation initiation factor 3 subunit J, eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 37kDa
NCBI: 8669
UniProt: O75822
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EIF3J antibody (23-694) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.