Western blot analysis of extracts of various cell lines, using BAZ1B antibody (23-942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (23-942) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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