BAZ1B Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-942
Artikelname: BAZ1B Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-942
Hersteller Artikelnummer: 23-942
Alternativnummer: PRS-23-942-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
Konjugation: Unconjugated
Alternative Synonym: Tyrosine-protein kinase BAZ1B, Bromodomain adjacent to zinc finger domain protein 1B, Williams syndrome transcription factor, Williams-Beuren syndrome chromosomal region 10 protein, Williams-Beuren syndrome chromosomal region 9 protein, hWALp2, BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 190kDa
NCBI: 9031
UniProt: Q9UIG0
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using BAZ1B antibody (23-942) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (23-942) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BAZ1B antibody (23-942) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.