Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175) at 1:1000.
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.
Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175) at 1:1000 at each lane.
Antibody is used in Western blot to detect RYK in Jurkat cell lysate.
Western blot analysis of RYK using rabbit polyclonal RYK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma, HC = hepatocarcinoma.
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