AIFM2 (IN) Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	AIFM2 (IN) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-9655
Hersteller Artikelnummer:	9655
Alternativnummer:	PRS-9655-0.02,PRS-9655-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-AIFM2 antibody ( 9655) was raised against a peptide corresponding to 14 amino acids near the center of human AIFM2. The immunogen is located within 130-180 amino acids of AIFM2.
Konjugation:	Unconjugated
Alternative Synonym:	AIMF2

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 41kDObserved: 40 kD
NCBI:	84883
UniProt:	Q9BRQ8
Puffer:	AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 2 µg/mL, IHC: 1 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 4: Immunofluorescence Validation of AIFM2 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with 9655 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 5: Immunohistochemistry Validation of AIFM2 in Human BrainImmunohistochemical analysis of paraffin-embedded human brain tissue using anti-AIFM2 antibody (9655) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6: Immunohistochemistry Validation of AIFM2 in Mouse BrainImmunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AIFM2 antibody (9655) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7: Immunohistochemistry Validation of AIFM2 in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AIFM2 antibody (9655) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1: WB Validation in Human Mouse and Rat Cell LinesLoading: 15 &956,g of lysate. Antibodies: AIFM2 9655, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
	Figure 2: WB Validation in Mouse Skeletal MuscleLoading: 15 &956,g of lysate. Antibodies: AIFM2 9655, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
	Figure 3: WB Validation in Rat TissuesLoading: 15 &956,g of lysate. Antibodies: AIFM2 9655, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.