PEX10 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-9671
- Bilder (7)
| Artikelname: | PEX10 (CT) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-9671 |
| Hersteller Artikelnummer: | 9671 |
| Alternativnummer: | PRS-9671-0.02,PRS-9671-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10. The immunogen is located within last 50 amino acids of PEX10. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 4 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. |
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Figure 2 WB Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane.Antibodies: PEX10 9671, 4 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. |
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Figure 3 WB Validation in Rat TissuesLoading: 15 &956,g of lysates per lane.Antibodies: PEX10 9671, 4 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. |
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Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 6 Immunofluorescence Validation of PEX10 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 1 WB Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysate Antibodies: PEX10 9671, 4 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. |
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