Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
Konjugation:
Unconjugated
Alternative Synonym:
DDDDK epitope tag,DDDDK epitope tag,DYKDDDDK epitope tag
This is a Flag-Tag Tags and Controls Antibody from Reddot Biotech. This product is for Research Use Only.
Klonalität:
Monoclonal
Konzentration:
1mg/mL
Isotyp:
IgG2a
Puffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
1x10 6 CHO cells Transfected with a Flag plasmid were stained with 0.2ug Anti-Flag tag mAb(RD86725A) and AF647 conjugated Goat Anti-mouse IgG (H+L),lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Monoclonal Antibody at 1:2000 dilution.
Lane 1:HEK-293 transfected with DDDDK-tagged (input).Lane 2: Mouse monoclonal IgG2a instead of RD86725A in HEK-293T transfected with DDDDK-tagged.Lane 3: RD86725A IP in HEK-293T transfected with DDDDK-tagged.
Western blotting with anti-FLAG monoclonal antibody at dilution of 1:5000.lane 1: HEK 293 whole cell lysate, Lane 2: FLAG tag transfected HEK 293 cell lysates
Antibodies from Reddot Biotech
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
