This is a (KO Validated) CDK4 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
Klonalität:
Polyclonal
Konzentration:
1mg/mL
Molekulargewicht:
Observed MW: Refer to figures. Observed MW: Refer to figures
Puffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Immunofluorescence analysis of NIH/3T3 using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human colon using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.
Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of 293T cells using CDK4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and CDK4 knockout (KO) HeLa cells using CDK4 Polyclonal Antibody at 1:1000 dilution.
Antibodies from Reddot Biotech
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