This is a Akt(Phospho-Ser473) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Puffer:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from UV treated 293/3T3/Hela, using Phospho-Akt(Ser473) Antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide)
Immunofluorescence staining of methanol-fixed 293 cells untreated or treated with IFN using Akt (Phospho-Ser473) Antibody (RDSA62058).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Akt (Phospho-Ser473) Antibody (RDSA62058).
Western blotting analysis using Akt(Phospho-Ser473) Antibody (RDSA62058).
Western blot analysis of extracts of various cell lines, using Akt(Phospho-Ser473) (RDSA62058)
Western blot analysis of various lysates using Akt(Phospho-Ser473) Antibody at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution.Lysates/proteins: 25µg per lane.
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