Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Konjugation:
Unconjugated
Alternative Synonym:
CFL,CFL1
This is a cofilin(Phospho-Ser3) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Puffer:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody (RDSA62138)(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody (RDSA62138)(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody (RDSA62138).
Lane 1 : Hela treated with H2O2, Lane 2 : Mouse brain tissue, Lane 3 : Rat brain tissue, Lane 4 : N-peptide blocked Hela treated with H2O2, Lane 5 : N-peptide blocked Mouse brain tissue,Lane 6 : N-peptide blocked Rat brain tissue,Lane 7 : P-peptide blocked Hela treated with H2O2, Lane 8 : P-peptide blocked Mouse brain tissue,Lane 9 : P-peptide blocked Rat brain tissue,Lysates/proteins at 40 µg per lane.Predicted band size : 19 kDa Observed band size : 19 kDa
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