Source: Recombinant protein corresponding to Lys313~Leu618 from mouse Toll Like Receptor 5, fused to His-Tag & GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~64.7kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with dH2O to a concentration of 0.1-1.0mg/ml. Do not vortex. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder in 100mMNaHCO3, 500mM sodium chloride pH 8.3, containing 0.01% SKL, 5% Trehalose. Reconstitute with dH2O to a concentration of 0.1-1.0mg/ml. Do not vortex
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