Synthetic peptide corresponding to aa250-263, KLYYDNEKCWFGKR, from CRHR1. Species sequence homology: rat, canine, bovine and sheep.
Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:1000 Western Blot: 1ug/ml Immunohistochemistry (paraffin): 5ug/ml using Human Brain (Cortex) Immunofluorescence: 10ug/ml. Expression of the protein seen in the cytoplasm and vesicles of MCF7 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.