Axl, Recombinant, Human, GST-Tag (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)
Artikelnummer:
USB-A4775-10
Hersteller Artikelnummer:
A4775-10
Alternativnummer:
USB-A4775-10-10
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Human AXL (GenBank Accession No. NM_021913), (a.a. 473-894) with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 140 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30C. Assay Buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate, and 1ug/ml recombinant AXL. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
