CD55 is a 70kD glycoprotein also known as Decay Accelerating Factor (DAF). Distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. Found specifically at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. This antibody has a functional binding affinity to erythrocytes of 8.7x10e7 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Functional assays: Removal of sodium azide is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3H1564 BRIC 216]
Isotyp:
IgG1
Reinheit:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
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