Human CD55 is a GPI anchored protein that can protect cells from complement mediated lysis. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): acetone fixation. Flow Cytometry: 10ug/ml labels 5x10e5 HPB-MLT cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2410-09A at 10ug/ml. 2. Cells were washed twice and incubated with a goat x mouse 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.44 log10 fluorescent units when compared to a Mouse IgG1 negative control (I1904-78R) at a similar concentration. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[7H134]
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
