Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500-1:8000 Western Blot: 1:500-1:8000 Immunohistochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Reinheit:
Purified by affinity chromatography
Formulierung:
Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
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