Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kD and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Applications: Suitable for use in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by a multi step process which included delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis.
Formulierung:
Supplied as a liquid in 0.1M Tris HCl, 0.5M sodium chloride, pH 8.0, 0.09% sodium azide. No stabilizing proteins added.
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