IRAK 4 (IL-1 Receptor Associate Kinase 4) (HRP), Rabbit
Artikelnummer:
USB-I8600-17C-HRP
Hersteller Artikelnummer:
I8600-17C-HRP
Alternativnummer:
USB-I8600-17C-HRP-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IF, WB
Immunogen:
Synthetic peptide derived from the N-terminal region of the human IRAK4 protein. Species Sequence Homology: mouse and rat, 100%
(IRAKs) are important mediators in the signal transduction of the Toll/IL-1 receptor (TIR) family. Members of the TIL family play a role in innate immune responses by coordinating host defense mechanisms. IRAK4 is the most recently discovered IRAK protein. IRAK-4 has been shown to interact with IRAK-1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK-4 has been shown to activate NFkB as well as mitogen-activated protein (MAP) kinase pathways. Studies demonstrate that mice lacking IRAK4 are severely impaired in their IL-1, Toll-like receptor (TLR) signaling and response to viral and bacterial challenges, this indicates that IRAK4 has an important role in innate immunity. Humans lacking IRAK 4 exhibit pyogenic bacterial infections, while their blood and fibroblast cells fail to activate NF-kB. IRAK-4 is believed to be required for the LPS-induced activation of antigen-presenting cells. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Reinheit:
Purified by immunoaffinity chromatography
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
