Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165), Rabbit
Artikelnummer:
USB-I8601-01
Hersteller Artikelnummer:
I8601-01
Alternativnummer:
USB-I8601-01-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA
Immunogen:
Synthetic 17 amino acid peptide sequence from rat IRAP extracellular domain (KLH coupled).
IRAP (also known as placental leucine aminopeptidase, PLAP, leucyl cystinyl aminopeptidase, LNPEP, oxytocinase, Otase, vesicle protein of 165kD or vp165) belongs to the peptidase M1 family. IRAP (mouse 966aa, rat 1025 aa, human 1025 aa, chromosome 5q14.2-q15), a zinc-containing metalloprotein, degrades peptide hormones such as oxytocin, vasopressin, and angiotensin III, and inactivates Met-enkephalin and dynorphin. It is a type 2 membrane protein and is secreted due to proteolytic processing. IRAP is glycosylated and alternatively spliced to at least 3 isoform (1-15 aa missing in isoform 2 and 1-20 missing in isoform 3). The N-terminus (1-110 aa) is located in the cytoplasm, followed by TM domain (111-131 aa), the C-terminus (132-1025 aa) is extracellular. The secreted form is cleaved at 154-155 aa (processed secreted form 155-1025 aa). IRAP is highly expressed in placenta, heart, kidney, and small intestine. In brain, only the membrane form is found. The brain form is found to be 140kD due to differential glycosylation. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: I8601-01A: Insulin Regulated Aminopeptidase, Rat, Control Peptide Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
